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Load the desired volume (e.g. 35 µL) of samples per well in a 10% polyacrylamide gel. Run SDS-PAGE for 30 minutes at 200 V. Rinse gel twice with distilled water. Incubate gel in
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Novex DNA Retardation Gels consist of 6% polyacrylamide prepared with 0.5X TBE as the gel buffer. They provide good resolution of 60–2,500 bp DNA fragments. 0.5X TBE buffer offers good fragment separation in
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Nonionic polyacrylamide is combined with lignocellulose, and certain chemical additives are added, and it is widely used as a water shutoff agent in oilfields. (6) Soil moisturizer Conserve water and fix sand in the soil. Planting grass and trees on hillsides can act as a moisturizer on sand fixation and dust prevention.
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Polyacrylamide Gel Electrophoresis (PAGE)
The gels typically consist of acrylamide, bisacrylamide, the optional denaturant (SDS or urea), and a buffer with an adjusted pH. The ratio of bisacrylamide to acrylamide can be varied for special purposes,
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Running agarose and polyacrylamide gels IDT
Agarose gels can be used to resolve large fragments of DNA. Polyacrylamide gels are used to separate shorter nucleic acids, generally in the range of 1−1000 base pairs, based on the concentration used
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Properties of Polyacrylamides polymerdatabase
The only commercially important polyacrylamide is poly (2-propenamide) which is simply called polyacrylamide or PAM [-CH 2 CH (CONH 2 )-]. It is a non-ionic, watersoluble, and biocompatible polymer that can be tailored to meet a broad range of applications. The polymer can be synthesized as a simple linear chain or as a cross
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The role of hydration degree of cations and anions on the
The polyacrylamide is a linear polymer made of acrylamide monomers ( Fig. 1 a ). Due to their manufacturing process, nonionic polyacrylamides usually have a small degree of hydrolysis (less than 0.1% acrylic acid, Fig. 1 b). Download : Download full-size image Fig. 1. Chemical structure of the monomers composing the nonionic polyacrylamide. 2.2.
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