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Jul 07, 2017 · Following formation of the separating gel, the WGA-polyacrylamide gel solution was made by mixing 0.375 ml of a 10 mg/ml WGA solution, 0.25 ml of a 30%
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After 10- or 30-min incubation with cellulose, 2 μL of the unbound and cellulose-bound fractions was loaded onto a non-denaturing 2.2% agarose gel and a native 21% polyacrylamide gel. To visualize the dsRNA, the agarose gel contained 0.005% (v/v) GelRed, while the polyacrylamide gel was stained post-run with SYBR Gold (Thermo
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Jul 07, 2017 · Following formation of the separating gel, the WGA-polyacrylamide gel solution was made by mixing 0.375 ml of a 10 mg/ml WGA solution, 0.25 ml of a 30% acrylamide solution, 0.25 ml of the stock separating gel buffer, 0.119 ml of deionized water, 4.5 μl of 10% APS and 1.5 μl of TEMED, and was poured on top of the
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Steps Warnings Materials Metadata Metrics Abstract Parameters adjusted for gels 7 cm and 1.5 mm thick. Before starting Organize your workspace Make sure all solutions and
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Jul 07, 2017 · Following formation of the separating gel, the WGA-polyacrylamide gel solution was made by mixing 0.375 ml of a 10 mg/ml WGA solution, 0.25 ml of a 30% acrylamide solution, 0.25 ml of the stock separating gel buffer, 0.119 ml of deionized water, 4.5 μl of 10% APS and 1.5 μl of TEMED, and was poured on top of the separating gel.
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